Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Evaluation of α-Glucosidase Inhibitory Activity of Dichloromethane and Methanol Extracts of Croton bonplandianum Baill

Muhammad Naeem Qaisar , Bashir Ahmad Chaudhary, Muhammad Uzair Sajid, Nawaz Hussain

Department of Pharmacy, Bahauddun Zakariya University, Multan, Pakistan;

For correspondence:- Muhammad Qaisar Email: naeemqaisar78@uos.edu.pk Tel:+0092619210089

Received: 25 February 2014 Accepted: 19 September 2014 Published: 24 November 2014

Citation: Qaisar MN, Chaudhary BA, Sajid MU, Hussain N. Evaluation of α-Glucosidase Inhibitory Activity of Dichloromethane and Methanol Extracts of Croton bonplandianum Baill. Trop J Pharm Res 2014; 13(11):1833-1836 doi: 10.4314/tjpr.v13i11.9

© 2014 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To evaluate the α-glucosidase inhibitory activity of Croton bonplandianum Baill as a probable remedy for the management of diabetes.

Methods: Dichloromethane and methanol extracts were prepared and screened for their α-glucosidae inhibitory activity using standard in vitro α-glucosidae inhibition assay. Acarbose was used as positive control.

Results: α-Glucosidae inhibition activity of dichloromethane extract was 97.89 % with 50 % inhibitory concentration (IC₅₀) of 14.93 mg/ml, while the methanol extract was not active. Acarbose (reference) exhibited 92.23 % inhibition with IC₅₀ of 38.25 mg/ml.

Conclusion: The plant contains bioactive compounds with enzyme-inhibiting activity, and thus lends some support for the traditional use of this herb in the management of diabetes. Dichloromethan extract has higher α-glucosidase inhibition activity than the methanol extract. However, further investigation of the plant, including identification of its active components are required.

Keywords: ^5;-Glucosidase, Inhibition, Diabetes, Acarbose, Croton bonplandianum